Graphene based gene transfection

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August undefined, 2024

WebApr 1, 2024 · Delivery of functional genes into stem cells shows great application prospect in DNA-based tissue engineering. However, comparing with epithelial cells and cancer cells, stem cells usually exhibit low gene transfection efficiency. To enhance the transfection efficiency, non-viral gene delivery in co …

Facile Synthesis of N-Doped Graphene Quantum Dots as Novel …

WebOct 13, 2014 · Graphene based biosensors make use of two strategies-1) probe molecule on graphene interacts with the analyte (FRET based ... Zhang S, Liu Z (2011) Graphene based gene transfection. Nanoscale 3: 1252-1257. Zhou L, Wang W, Tang J, Zhou JH, Jiang HJ, et al. (2011) Graphene oxide noncovalent photosensitizer and its anticancer … WebJan 23, 2024 · Recently graphene has been used in biomedical applications including DNA sequencing 10, biosensor development 11, and graphene-enhanced cell differentiation … city car driving lite download

Graphene: A Multifaceted Nanomaterial for Cutting Edge …

WebTo evaluate the transfection efficiency of NGQDs, we delivered mRNA into HeLa cells with NGQDs. As shown in Figure 4 a, the NGQDs formed complexes with mRNA at a 20:1 … WebDec 4, 2024 · The objective of this study is to design a graphene-based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene delivery system is developed using polyethylenimine (PEI) functionalized graphene oxide (GO) complex loaded with miR-7b overexpression plasmid. WebDec 4, 2024 · The objective of this study is to design a graphene-based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene … city car driving logitech g920

Graphene materials as 2D non-viral gene transfer vector platforms …

Graphene-based Nano-Carrier modifications for gene …

WebJul 30, 2024 · Methods: Graphene oxide/APTES was modified by spermine (GOAS) and characterized by FT-IR, DLS, SEM and AFM techniques. Then pEGFP-p53 was loaded on GOAS, transfected into cells and evaluated by fluorescent microscopy and gene expression techniques. Results: FT-IR data approved the GOAS sheet formation. Ninety percent of … WebJan 10, 2014 · Successful gene therapy requires efficient and safe gene vectors that protect DNA from nuclease degradation as well as facilitate DNA uptake with high transfection efficiency [139], [140]. Graphene has been explored for applications in gene delivery, gene–drug co-delivery and protein delivery with good supportive literature available. city car driving magyarositásWebJun 1, 2014 · Abstract. Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also … dick\u0027s sporting goods retail associate pay

"WebApr 11, 2024 · PEI-functionalized graphene-based transporters have been widely used in gene therapy for various illnesses, including myocardial infarction, osteoporosis [188], and tumors [189]. Other agents, in addition to PEI, have been used to alter graphene-based resources for improved transfection, such as chitosan [ 173 , 184 , [190] , [191] , [192 ... " - Graphene based gene transfection

Graphene based gene transfection

Graphene-based Nano-Carrier modifications for gene …

WebJul 1, 2024 · Fig. 1. Illustration of various carbon-based structures (graphite sheets, graphene, nanotubes, fullerene, and nanodiamond) that are frequently used in medicine and biomedical applications. 2. Gene therapy and carbon structures. Gene therapy simply means using therapeutic nucleic acids as drugs to treat diseases [7]. WebJan 27, 2011 · Degradable copolymer based on amphiphilic N-octyl-N-quatenary chitosan and low-molecular weight polyethylenimine for gene delivery. Liu C , Zhu Q , Wu W , Xu …

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WebIn general, it can be concluded that polymer-functionalized graphene-based carriers have been widely applied in gene delivery to treat various diseases including cancer, 251 osteoporosis, 252 and myocardial infarction. 253 A variety of macromolecules including cationic polymers, dendrimers, chitosan, and peptides have been used to modify ... WebJan 15, 2024 · Graphene and graphene-based nanomaterials such as graphene oxide (GO), reduced graphene oxide (rGO) and graphene quantum dots (GQDs) ... Feng, S. Zhang and Z. Liu, Graphene based gene transfection, Nanoscale 3 (2011) 1252–1257.

WebMany researchers have tried to transfect genes using carbon-based materials such as graphene oxides (GOs) and graphene quantum dots (GQDs) [29,30,31,32]. ... Prior to in vitro gene transfection with NGQDs, we verified the cytotoxicity of the NGQDs via a CCK-8 assay kit. For the CCK-8 assay, various concentrations of NGQDs from 1 μg/mL to 1000 ... WebWe conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform transfection and overall increased efficiency. ... Liu, Z. Graphene based gene transfection. Nanoscale 2011, 3, 1252–1257. [Google Scholar] Liu, Z.; Robinson, J.T.; Sun, X.; Dai ...

WebApr 12, 2024 · Polymeric gene carriers target the nucleus and express their therapeusis through transcription and translation . Gene carriers are either viral vectors (retro-viruses and adenoviruses) or non-viral vectors. Viral vectors submit their genetic materials to the host cells and are known to attain greater transfection . 18.3.1.2 Polymeric Micelles WebDec 7, 2024 · Graphene has been widely studied for biomedical use since its first appearance in 2004 [3], partly owing to its high Young's modulus, high fracture strength, high electrical conductivity, and excellent optical performance. Graphene also possesses a large specific surface area for adsorption of bioactive compounds and has high optical …

WebJan 6, 2024 · Graphene oxide for transfection. ThermoFisher Scientific describes transfection as the process of artificially introducing nucleic acids – DNA or RNA – into cells, utilising means other than viral infection. A paper published in Nanoscale 2011 titled ‘Graphene based gene transfection’ showed that graphene oxide (“GO”) bound with ...

WebFeb 28, 2024 · Feng L Zhang S Liu Z Graphene based gene transfection Nanoscale 2011 33 1252 1257 21270989 , [Web of Science ®], [Google Scholar] Yang X Wang Y Huang X Multi-functionalized graphene oxide based anticancer drug-carrier with dual-targeting function and pH-sensitivity J Mater Chem 2011 2110 3448 3454 , [Web of Science ®], … dick\u0027s sporting goods reston vaWebGraphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we … city car driving macWebVisualizing The Localization Of Transfection Complexes During Graphene Nanoparticle-Based Transfection Journal of Materials Chemistry B … city car driving logitech g923WebMar 1, 2011 · Graphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for … city car driving map dowloadWebNov 22, 2016 · Graphene based gene transfection. Nanoscale 2011; 3: 1252–1257. Article CAS Google Scholar Prencipe G, Tabakman SM, Welsher K, Liu Z, Goodwin AP, Zhang L et al. PEG branched polymer for ... city car driving manualWebGraphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we … city car driving m5 e60WebJan 27, 2011 · Our results suggest graphene to be a novel gene delivery nano-vector with low cytotoxicity and high transfection efficiency, promising for future applications in non … dick\\u0027s sporting goods return label