WebJun 5, 2024 · By cycloheximide assay, we provide evidence that GSKJ4 reduces CREB protein stability; moreover, proteasome inhibition largely counteracts the GSKJ4-induced CREB downregulation. Very interestingly, a rapid CREB phosphorylation at the Ser133 residue precedes CREB protein decrease in response to GSKJ4 treatment. In addition, … WebJul 27, 2015 · Protein stability assay. HeLa and NSC34 cells were co-transfected with equal amount of pcDNA4/a-SMN and pcDNA4/FL-SMN. After 20 hrs cells were treated with 100 μg/ml cycloheximide (CHX, Calbiochem, Darmstadt, Germany) to inhibit protein synthesis. ... or cycloheximide, to block protein translation and allow mRNA …
Analysis of Protein Stability by the Cycloheximide Chase …
WebProtein stability influences many aspects of biology, and measuring their stability in vivo can provide important insights into biological systems.This chapter describes in details two methods to assess the stability of a specific protein based on its transient expression in Arabidopsis protoplasts. … WebJan 1, 2015 · Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in … shelly user password
Cycloheximide chase - Wikipedia
WebApr 4, 2024 · Besides, NEDD4L signals were also detected in the protein complex pulled down by the anti-GPX-specific antibody. Moreover, a cycloheximide experiment was conducted to analyze GPX4 protein stability in NEDD4L-KO (knock-out) KGN cells. As shown in Fig. 5B and andC, C, GPX4 protein stability was markedly increased in … WebJan 15, 2024 · Promoter-based luciferase assays further confirmed that, ... To understand if the decreased AGR2 by MG132 is caused by protein stability, cycloheximide (CHX), a protein synthesis inhibitor, was ... WebNov 10, 2024 · Protein stability assays. After 24 h of culture in a 6-well plate, cycloheximide (CHX, Sigma-Aldrich) was added to each well at a concentration of 50 … sports car trailer for corvettes