WebJul 27, 2015 · Cycloheximide-treated cells were harvested at different time points (0, 1, 3, 5 and 7 hrs) and processed for immunoblotting with anti-tag antibody (anti-Xpress, Life Technologies). Anti-actin antibody was used as internal controls. WebIn the cycloheximide chase experiment, we analyzed Cdc25A protein turnover in the presence and absence of USP29. Our results demonstrated that the half-life of Cdc25A is around 20–30 min (Figure 3B). However, the overexpression of USP29 prolonged the half-life of the Cdc25A protein ...
PKD phosphorylation and COP9/Signalosome modulate …
WebOver the years, procedures to determine the half-life of proteins in cultured eukaryotic cells have been well-established. This chapter describes in detail the two most frequently … WebJul 25, 2024 · The protein half-life of YAP was analyzed by Cycloheximide assay, and the TEAD activity was detected by Luciferase reporter assays. Results: Clinical sample of ESCC revealed that low PARK2 expression correlated with late tumor stage (P < 0.001), poor differentiation (P < 0.04), lymph node (P < 0.001) and distant metastasis (P = 0.0087). black-and-white signature logo
Analysis of Protein Stability by the Cycloheximide Chase Assay.
WebApr 14, 2024 · Western blot results showed that CYP1B1 overexpression decreased the half-life of ACSL4 (Fig. 2G). Based on these results, we considered that CYP1B1 decreased ACSL4 protein stability by increasing ... WebThereafter the cells were treated with cycloheximide (CHX) to stop translation, and protein extracts were prepared at different time points after treatment (0 h, 1 h, 2 h, ... Reports on the half-life of RBPJ protein vary dramatically depending on cell type and setting. For example, a rapid turnover of RBPJ within 2 h, ... WebAug 29, 2006 · These comparisons further suggest that, in most cases, the effect of cycloheximide on half-life is secondary to other sources of noise in our experiments. In summary, we have generated a large-scale collection of protein half-life measurements. black and white siberian tiger