Ctd110.6 antibody
WebJan 1, 2015 · The specificity of the CTD110.6 antibody immunoreactivity was confirmed by a hapten inhibition test using 0.1 m GlcNAc. B, the levels of O-GlcNAc modification on N1EGF1–36-MycHis quantitated by densitometric analyses of immunoblots represented in A (n = 3; results are shown as mean ± S.D.; *, p < 0.01; **, p < 0.05; unpaired Student's t … WebOct 11, 2013 · For immunodetection, CTD110.6 antibody was used because it is widely used for the detection of O-GlcNAcylated proteins. Compared to the α-DG-Fc isolated from the control transfectants, immunoreactivity of α-DG-Fc towards CTD110.6 was elevated upon GTDC2 overexpression (Fig. 1A).
Ctd110.6 antibody
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WebPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. WebJan 13, 2003 · Anti-CD46 antibodies inhibit binding of Ad7 and Ad11 virions to CHO-BC1 cells. In a second experiment, we preincubated A549 cells or CHO-BC1 cells with …
WebImmunofluorescence staining of CTD110.6 on methanol fixed HeLa cells at 10 µg/ml. Following fixation, the cells were incubated with the primary antibody for one hour at …
WebClone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The presence of GlcNAc, but not GalNAc, abolished the detection of the target modification. Clone CTD110.6 did not recognize peptides with unmodified serine and threonine residues (Comer, F.I., et al. (2001). WebApr 18, 2014 · Binding by antibodies CTD110.6, #3, and #10 was inhibited by 5 m m GlcNAc, but not 5 m m GalNAc, and was thus specific for recognition of O-GlcNAc. However, binding of antibodies 10D8, RL2, and #14 was not inhibited by the presence of 5 m m GlcNAc. Therefore, only antibodies CTD110.6, #3, and #10 should be used for cell …
WebMay 18, 2024 · 3. Incubate the blots with CTD110.6 (diluted to 1 μg/ml in antibody dilution buffer), in duplicate, with and without 100 mM GlcNAc, overnight at 4°C. An antibody that has been antigen purified using a GlcNAc-agarose column yields higher-quality blots. Commercially available CTD110.6 is purified by ammonium sulfate precipitation.
WebFigure 6 HRECs exposed to TNF-alpha, and high glucose for 24 h demonstrated alterations in VE-Cadherin and defective complex formation. The whole cell lysates were immunoprecipitated with indicated antibodies, and the immunocomplexes were analyzed for O-GlcNAcylation using anti-O-GlcNAc antibody (clone CTD110.6) ( A ). how many protons are there in copperWebMar 5, 2024 · Compared to immunostaining of O-GlcNAcylated proteins by CTD110.6 antibody, OGT immunostaining appears to be more cytosolic with less intense immunoreactivity in hippocampal CA1 (ratios of immunoreactivity intensity between CA1 to adjacent region are ~ 0.73 for OGT and ~ 2.5 for CTD110.6, respectively) or dentate … how cross out text in outlookWebwww.ncbi.nlm.nih.gov how crop image in illustratorhttp://bb-china.net/search.html?brand=42&pcategory=0&classd=0&use=0&kw=&promotions=2&inventory=2&category=17&page=21 how crop pdf fileWebApr 24, 2024 · Among these antibodies, CTD110.6 and RL2 are the most widely used. However, due to a certain degree of amino acid sequence dependency, these antibodies cannot detect all proteins with O-GlcNAcylation. Additionally, a few antibodies for site-specific protein O-GlcNAcylation have been reported, ... how many protons are there in one atom of 14nWebat 6 h after the treatment, i.e. at the same time that an increase was previously noted for proteins identified by their reaction with CTD110.6 antibodies (Figure 1b). The immunoprecipitation analysis showed that CTD110.6 antibodies reacted with all four of the lower molecular weight forms that were immunoprecipitat- how many protons are there in radiumWeb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 how crop in illustrator